420+ Unique Proteins for Drug Development

Technical Details

To create the VirD array, 500+ unique human membrane protein open reading frames (ORFs) are modified to remove their stop codons and subcloned into the UL27 locus of the herpes simplex (HSV-1) virus genome with a C-terminal v5 tag. These individually engineered viron clones are then used to infect mammalian cells. Native human protein assembly machinery correctly folds the proteins and shuttles them to the viral membrane. Membrane-protein bearing VirD virions are concentrated from culture supernatants and their membrane-protein expression confirmed via anti-V5 immunoblots.

Purified virions were then printed on SuperEpoxy glass slides to create the final GPCR-VirD arrays. The quality of printed VirD-GPCR arrays is confirmed via staining for HSV-1 Glycoprotein D (gD) via anti-gD antibody and comparing those as compared with bovine serum albumin negative controls (BSA).

Service Details and Data Deliverables

A simple antibody interaction VirD service involves drug candidate antibodies diluted and reacted with individual microarrays with BSA blocking. After incubation, samples are stained for one or two desired fluorescent secondary detection antibodies (i.e., IgG1). Arrays are scanned and raw TIF images are created using Molecular Devices Genepix microarray scanners. Spots are then aligned to CDI library (.gal) files, and spot readings are output as raw Genepix results (.gpr) files. These raw results are then delivered both as .gpr files and combined output data tables.

Sample Requirements

Antibody isolates10 μg per sample (concentration 0.1 mg/mL)

Protein / peptide / small molecule10 μg per sample (concentration 0.1 mg/mL)

RNA1 mL per sample (concentratiion 2 μM)

Other fluids> Please contact us

Note

Human serum or plasma often contains anti-HSV-1 antibodies making VirD inappropriate for clinical studies.

Sample Shipping and Sample Return

We will include shipping details in your quote – you must cover the cost of shipping samples to CDI Labs. Typically, after you receive your report, CDI Labs keeps the remaining samples for two months and then disposes of them. When shipping to us, please let us know if you want the remaining samples returned after the study is complete. Return shipping will be charged.