CDI Labs Services for

Antibody Specificity Validation

HuProt tests and validates that the antibody recognizes its intended target with high specificity.

HuProt Validates Antibody Specificity

Validates that antibodies' recognize its intended target with high specificity while exhibiting minimal cross-reactivity with unrelated proteins or molecules

Monoclonal antibody specificity validation is a crucial step in antibody development. Specificity testing validates that the antibody recognizes its intended target with high specificity while exhibiting minimal cross-reactivity with unrelated proteins or molecules.

In the featured publication, HuProt™ microarray was used as the primary validation tool to identify mAbs with high specificity for their cognate targets. A total of 122,662 antibodies were tested using 2 x 7 array format mini-chips, and subsequently 5,882 antibodies were tested on HuProt. The entire pipeline identified 1,406 highly validated immunoprecipitation- and/or immunoblotting-grade mouse monoclonal antibodies against 737 human transcription factors.

Featured Publication

A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors

Anand Venkataraman, Seth Blackshaw, et. al.

Abstract

A key component of efforts to address the reproducibility crisis in biomedical research is the development of rigorously validated and renewable protein-affinity reagents. As part of the US National Institutes of Health (NIH) Protein Capture Reagents Program (PCRP), we have generated a collection of 1,406 highly validated immunoprecipitation- and/or immunoblotting-grade mouse monoclonal antibodies (mAbs) to 737 human transcription factors, using an integrated production and validation pipeline. We used HuProt human protein microarrays as a primary validation tool to identify mAbs with high specificity for their cognate targets.

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CDI Labs Advantages

The most comprehensive protein collections available allow for rapid proteome wide antibody repertoire analysis and target identification studies.

HuProt™ Proteome Microarrays provide high quality, reproducible results with a protein purification process that only selects properly folded proteins.

GMP/GLP compliant workflows and highly trained technicians ensure the highest quality results. Compliant data management processes ensure data integrity and security.

Cutting-edge synthetic biology platforms and an innovative team with deep proteomics bench expertise make CDI Labs an ideal CRO partner for even your most challenging projects.