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HuProt™ Human Proteome Microarray
The Most Comprehensive Collection of Full-length Proteins Available
With over 21,000+ GST-purified proteins, there has never been a protein library as comprehensive or thoroughly validated as HuProt™.
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The Broadest Coverage of the Human Proteome
HuProt consists of over 21,000+ unique human proteins, isoform variants and protein fragments – covering 16,794 unique genes, it is the most comprehensive collection of human proteins available.
Our industry-leading HuProt microarray includes 15,889 of the 19,613 canonical human proteins described in Human Protein Atlas, with broad coverage across protein subclasses.
Add your own custom proteins to HuProt - on the rare occasion when HuProt does not contain a protein that is of interest to you, we can add that protein(s) for a small additional fee.
Content includes major functional classes such as intracellular proteins, membrane proteins, enzymes, secreted proteins, transcription factors, transporters, GPCRs, cytokines, immune receptors, immune checkpoints, CD markers, ion channels, cytosolic proteins, nuclear receptors. Additionally there is thorough coverage for proteins enriched in major tissues of interest such as testis, cerebral cortex, thyroid gland, skin, fallopian tube, liver, parathyroid, intestine, kidney, spleen, muscle, epididymis, lymph node, bone marrow, adrenal gland, esophagus, heart, appendix, tonsil, prostate, rectum, adipose tissue, stomach, colon, cervix, uterus, gallbladder, seminal vesicle, breast, ovary, endometrium, smooth muscle, salivary gland, pancreas, and bladder.
Reproducible Protein Distribution
CDI Labs’ non-contact piezoelectric ‘inkjet’ process uses next-generation Arrayjet printers and allows for rapid production of high quality microarray slides time after time. In comparison to older contact pin printing methods – HuProt™ arrays are made with improved accuracy and reproducibility with excellent spot morphology.
Reproducible Serum and Plasma Profiling
Reproducible Proteome-Wide IgG Autoantibody Immunoprofiling of Healthy Human Pooled Plasma Within and Across HuProt Proteome Microarray Batches
Pooled human plasma was incubated on pairs of HuProt proteome microarrays across two print batches and stained with a fluorescent anti-IgG secondary antibody. Raw median fluorescence intensity data were captured, log2 transformed, and plotted. Linear regression analysis demonstrates reproducibility across the array pair from Lot 1 (R2 = 0.96), Lot 2 ( R2 = 0.92), and the averaged data from both array batches (R2 = 0.96). These results demonstrate the robust reproducibility of HuProt autoantibody seromics data both within and across microarray production lots.
Correctly-folded, Full-length Proteins in Their Native Conformation
Protein purification prior to microarray manufacturing selects for proteins with correctly-folded GST-fusion proteins. Testing on the HuProt Microarray demonstrates that the proteins are folded in native conformation and retain function (Hu S. poster; Venkataraman A., et al., 2018). When both native and denatured HuProt microarrays were probed with monoclonal antibodies that selectively recognize either linear or folded epitopes of their cognate antigen, the antibodies were found to recognize the appropriate antigen form (Venkataraman A., et al., 2018). Further tests (RNA binding) demonstrated that proteins on HuProt do retain function (Venkataraman A., et al., 2018).
- Production technology: Non-contact piezoelectric inkjet
- Slide type: Coated glass (PATH)
- Slide dimensions: 75 x 25 x 1 mm
- Detection method: Fluorescence
- Content: Recombinant human and mouse proteins
HuProt™ Focused Arrays
High-throughput Autoantibody Signature Validation
We also offer high-throughput autoantibody signature validation services using focused arrays. Autoantibody biomarkers targeting up to 300 proteins discovered using HuProt can be arrayed in a format optimized for the size of your biomarker set and throughput requirements. This service offers a fast and affordable solution for biomarker validation. We can synthesize individual proteins at scale for high-throughput screening and other applications.
CONTACT USfor more details on our focused array services.
Technical Doc
PDF | 2.47 MB
HuProt™ Human Proteome Microarray
User Manual for HuProt™ Human Proteome Microarray - VER...
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CSV | 4.00 MB
HuProt v3.0 Full Content
HuProt v3.0, has columns: block, row, column, system ID, symbol, categories, reviewed UniProt, unreviewed UnProt, CCDS, RefSeq,...
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CSV | 4.28 MB
HuProt v3.1 Full Content
HuProt v3.1, has columns: block, row, column, system ID, symbol, categories, reviewed UniProt, unreviewed UnProt, CCDS, RefSeq,...
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ZIP | 14.90 MB
HuProt v4.0 Full Content with Sequences
HuProt v4.0, has columns: system ID, symbol, nucleotide, protein, reviewed UniProt, unreviewed UnProt, CCDS, RefSeq, Ensembl, synonyms, categories. Zip file containing .tsv...
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ZIP | 430.74 KB
HuProt v4.0 .gal File
HuProt v4.0 standard .gal file for analysis of purchased array. Zip file containing .gal...
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ZIP | 17.60 MB
HuProt HPA Alignment
Human Protein Atlas Overlap to HuProt. For ontology enrichment analysis, subcellular localization information. Nomenclature alignment done using new BLAST results file (ZIP file containing...
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ZIP | 10.73 MB
HuProt BLAST Nomenclature
HuProt Sequence BLAST Label Curation vs. UniProt (Human and Mouse). No sequences are altered; label modernization of gene-names only (ZIP fiile containing...
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PDF | 4.01 MB
Empowering Discoveries in Disease Research
Main CDI Labs brochure containing overview of Autoantibody Seromics, information on HuProt™ Proteome Microarray, HuProt™ Focused Array, and VirD™ Membrane Protein Functional...
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PDF | 1.17 MB
HuProt™ Human Proteome Microarray for PROTAC and Molecular Glue Target...
Targeting "undruggable" proteins is a major frontier in drug discovery, aiming to address disease-associated proteins that lack traditional druggable sites, such as active sites for inhibitors....
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PDF | 2.83 MB
HuProt™ Proteome Microarray Full-length, Folded, GST-purified 3D...
HuProt™ Human Proteome Microarrays are fabricated by immobilizing 21,000+ individually purified GST-fused human proteins on a single glass slide. Unlike nucleic acids, many proteins can become...
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A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription...
HuProt Antibody Specificity: AA key component of efforts to address the reproducibility crisis in biomedical research is the development of rigorously validated and renewable protein-affinity...
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Autoimmunity in Down’s syndrome via cytokines, CD4 T cells and CD11c+ B...
Down’s syndrome (DS) presents with a constellation of cardiac, neurocognitive and growth impairments. Individuals with DS are also prone to severe infections and autoimmunity including...
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Baseline Serum Autoantibody Signatures Predict Recurrence and Toxicity in Melanoma Patients Receiving...
Adjuvant immunotherapy produces durable benefit for patients with resected melanoma, but many develop recurrence and/or immune-related adverse events (irAE). We investigated whether baseline...
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Autoantibodies against type I IFNs in humans with alternative NF-κB pathway...
HuProt Autoantibodies: Patients with autoimmune polyendocrinopathy syndrome type 1 (APS-1) caused by autosomal recessive AIRE deficiency produce autoantibodies that neutralize type I interferons...
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Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy...
HuProt Autoantibodies: Autoantibodies are present in healthy individuals and altered in chronic diseases. We used repeated samples collected from participants in the NYU Women’s Health Study to...
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Utility of Protein Microarrays for Detection of Classified and Novel Antibodies in Autoimmune...
Neural antibodies are detected by tissue-based indirect immunofluorescence assay (IFA) in Mayo Clinic's Neuroimmunology Laboratory practice, but the process of characterizing and validating novel...
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IgA transcytosis and antigen recognition govern ovarian cancer...
Most ovarian cancers are infiltrated by prognostically relevant activated T cells, yet exhibit low response rates to immune checkpoint inhibitors. Memory B cell and plasma cell infiltrates have...
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A causal link between autoantibodies and neurological symptoms in long...
Acute SARS-CoV-2 infection triggers the generation of diverse and functional autoantibodies (AABs), even after mild cases. Persistently elevated autoantibodies have been found in some individuals...
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Overview of HuProt™ Human Proteome Microarray
This video / animation is a short overview of the CDI Labs HuProt™ Human Proteome Microarray product and...
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Overview of Autoantibody Seromics
This video / animation is a short overview of CDI Labs Autoantibody...
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Discovering Biomarkers, Cross-Reacting Antibodies, and...
In this presentation, Heng Zhu, professor in the Department of Pharmacology and Molecular Sciences at the Johns Hopkins School of Medicine, will provide brief history of proteome array...
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Advancing Immuno-oncology Research with the HuProt™ Human Proteome...
In this video, Tyler Hulett, PhD, CSO at CDI Labs, highlights how the HuProt™ Human Proteome Microarray is advancing the field of autoantibody seromics and providing new insights into our...
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Discovering Novel Immune Biomarkers and Druggable Targets with...
In this webinar, Tyler Hulett, PhD, describes how seromics, the study of the total secreted antibody repertoire in serum and other bodily fluids, is traditional serology reborn in...
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Seromics: A New Frontier of Immune Biomarkers and Druggable Target...
It is already clear that each individual’s serome is incredibly unique, complex, and yet accessible via proteome microarrays (HuProt), phage-display immunoprecipitation sequencing (PhIP-Seq,...
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General Procedures: HuProt™ Human Proteome...
Series of videos that show users how to stain and prepare HuProt arrays. Procedures include Blocking Stage, Sample Preparation (for non-Seromics assays), Adding Samples to HuProt, Washing Stage,...
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