Antibody Pairs

We Specialize in the Development of High-performance Antibody Pairs

Deep domain expertise and advanced technologies to meet the demands of modern research and healthcare

Antibody pairs are fundamental tools in both research and therapeutic applications. These paired antibodies, typically configured as a capture antibody and a detection antibody, work together to provide specificity, sensitivity, and reliability in various biological and clinical processes. We have leveraged our proteome-wide human protein expression clone collection and our HuProt specificity screening to develop a streamlined and scalable workflow for the delivery of high-quality antibody pairs. As a result, we can efficiently identify antibody pairs with minimal cross-reactivity, reducing noise in multiplex assays. Our streamlined workflow delivers pairs for up to 15 antigens within 30 days, with the capability to scale production to meet your needs. Whether you're looking to enhance or customize assay content, we provide comprehensive support—from antibody development and characterization to the creation of antibody pairs. This efficient pipeline ensures we help you stay ahead with high-quality, low-noise solutions.

Developing effective antibody pairs is a complex and typically resource-intensive process that requires careful consideration of multiple factors to achieve specificity, sensitivity, and reproducibility. Challenges include:

Epitope Selection and Compatibility

• Non-overlapping Binding Sites: The antibodies must bind distinct, non-overlapping epitopes on the target molecule. Overlapping binding sites can lead to competitive binding, reducing assay performance.
• Epitope Availability: The structural conformation of the target may limit the accessibility of certain epitopes, making it difficult to identify compatible antibody pairs.

Target Complexity

• Multidomain Proteins: Targets with multiple domains or post-translational modifications can present challenges in identifying antibodies that bind specifically to the intended region.
• Dynamic Structures: Targets with conformational flexibility or aggregation tendencies can affect antibody binding.

Affinity Mismatch

• An imbalance in the binding affinities of the capture and detection antibodies can compromise the overall sensitivity and robustness of the assay.

Cross-reactivity

• Antibodies may inadvertently bind to unrelated molecules with similar epitopes, leading to false positives and reduced assay specificity.

With deep domain expertise, advanced technologies, and robust validation methods, CDI Labs is an ideal partner for the development of high-performance antibody pairs that meet the demands of modern research and healthcare applications.