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HuProt™ Human Proteome Microarray
With more than 21,000 GST-purified proteins, there has never been a protein library as comprehensive or thoroughly validated as HuProt™.
REQUEST INFOOur industry-leading HuProt microarray includes 15,889 of the 19,613 canonical human proteins described in Human Protein Atlas, with broad coverage across protein subclasses.
Add your own custom proteins to HuProt - on the rare occasion when HuProt does not contain a protein that is of interest to you, we can add that protein(s) for a small additional fee.
Content includes major functional classes such as intracellular proteins, membrane proteins, enzymes, secreted proteins, transcription factors, transporters, GPCRs, cytokines, immune receptors, immune checkpoints, CD markers, ion channels, cytosolic proteins, nuclear receptors. Additionally there is thorough coverage for proteins enriched in major tissues of interest such as testis, cerebral cortex, thyroid gland, skin, fallopian tube, liver, parathyroid, intestine, kidney, spleen, muscle, epididymis, lymph node, bone marrow, adrenal gland, esophagus, heart, appendix, tonsil, prostate, rectum, adipose tissue, stomach, colon, cervix, uterus, gallbladder, seminal vesicle, breast, ovary, endometrium, smooth muscle, salivary gland, pancreas, and bladder.
CDI Labs’ non-contact piezoelectric ‘inkjet’ process uses next-generation Arrayjet printers and allows for rapid production of high quality microarray slides time after time. In comparison to older contact pin printing methods – HuProt™ arrays are made with improved accuracy and reproducibility with excellent spot morphology.
Pooled human plasma was incubated on pairs of HuProt proteome microarrays across two print batches and stained with a fluorescent anti-IgG secondary antibody. Raw median fluorescence intensity data were captured, log2 transformed, and plotted. Linear regression analysis demonstrates reproducibility across the array pair from Lot 1 (R2 = 0.96), Lot 2 ( R2 = 0.92), and the averaged data from both array batches (R2 = 0.96). These results demonstrate the robust reproducibility of HuProt autoantibody seromics data both within and across microarray production lots.
Protein purification prior to microarray manufacturing selects for proteins with correctly folded GST-fusion proteins. Testing on the HuProt Microarray demonstrates that the proteins are folded in native conformation and retain function (Hu S. poster; Venkataraman A., et al., 2018). When both native and denatured HuProt microarrays were probed with monoclonal antibodies that selectively recognize either linear or folded epitopes of their cognate antigen, the antibodies were found to recognize the appropriate antigen form (Venkataraman A., et al., 2018). Further tests (RNA binding) demonstrated that proteins on HuProt do retain function (Venkataraman A., et al., 2018).
We also offer high-throughput focused array services to validate autoantibody signatures discovered with the HuProt microarray. CONTACT USfor more details on our focused array services.